Letter: Samples for hepatitis B antigen testing.

EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN SACCHAROMYCES CEREVISIAE

The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 a...

Pitfall of hepatitis B surface antigen testing in a kidney transplant recipient presenting hepatitis B reactivation.

Diagnosis of hepatitis B virus (HBV) infection based on hepatitis B surface antigen (HBsAg) detection can be hampered in the setting of HBV reactivation in immunocompromized patients with prior serology indicating past cured infection, and can be associated with severe or fulminant and fatal hepatitis. We present a case of HBV reactivation in a renal transplant patient in whom HBsAg failed to b...

A multiple sampling device for the mass screening of serum samples for hepatitis B surface antigen.

Radioimmunoassay for hepatitis B core antigen.

Serum hepatitis B core antigen (HBcAg) is an important marker of hepatitis B virus replication. We describe an easy, sensitive radioimmunoassay for determination of HBcAg in detergent-treated serum pellets containing Dane particles. Components of a commercial kits for anti-core determination are used, and HBcAG is measured by competitive inhibition of binding of 125I-labeled antibodies to HBcAg...

Hepatitis B antigen in prostitutes.

exposure (Prince, Hargrove, Szmuness, Cherubin, Fontana, and Jeffries, 1970; Papaevangelou, Kourea, and Tsoukas, 1971; Fulford, Dane, Catterall, Woof, and Denning, 1973). A high frequency of HB Ag-positive hepatitis cases was also found among homosexuals (Vahrman, 1973) and evidence has been presented that hepatitis B may spread between sexual partners (Hersh, Melnick, Goyal, and Hollinger, 197...